As little as 50 ng of total RNA can be used as starting material. The first protocol employs long-distance PCR (LD PCR) for researchers limited by their starting material. This kit contains two separate protocols, allowing you to choose a method based on your starting material. The kit combines SMART technology ( Switching Mechanism At 5' end of RNA Template) for cDNA synthesis with adapter-free, directional cloning into the TriplEx2 vector. The SMART cDNA Library Construction Kit is designed for the cloning of full-length cDNA into a phage TriplEx2 vector. 100 ng of each purified DNA fragment were electrophoresed again at 100 V for 20 min (Figure 2).Our cDNA library construction kits utilize SMART technology to generate libraries enriched for full-length cDNA. The fragments were purified with the FastGene ® Gel/PCR Extraction Kit. The target fragments were excised out of the gel and transferred in a 1.5 mL tube. The 0.7% agarose gel was produced using 1x TAE buffer (Figure 1). The restriction digest was analysed by agarose gel electrophoresis at 100 V for 20 min. The customer also highlighted the fast preparation, easy handling, high recovery rate for large fragments and the unproblematic performance in downstream applications.Ī 6.9 kb large plasmid was digested with a restriction enzyme. In this AppNote the FastGene® Gel/PCR Extraction Kit was used for the isolation of two DNA bands after restriction digestion.īoth fragments show a good recovery rate after extraction. It is a well-known problem that the recovery of DNA fragments larger than 1 kb proves to be difficult and leads to the loss of large amounts of DNA. Lane 5,6 and 7: FastGene ® Gel/PCR Extraction Kit Lane 1: Unpurified PCR fragment M = Marker DNA was eluted in 20 µL elution buffer and 10 µL of eluate was analyzed on a 2% TAE agarose gel. After electrophoresis the DNA was purified using a competitor kit and the FastGene ® Gel/PCR Extraction Kit, according to manufacturers protocol. The DNA recovery from agarose gels using FastGene ® Gel/PCR Extraction Kits is very high even for small fragments.įigure: 10 µL of a PCR stock solution with a 100 bp amplicon were applied on a TBE agarose gel. 5 μL of eluted DNA were analyzed on a 1.5% TAE agarose gel. The figure demonstrates that the FastGene ® Gel/PCR Extraction Kit shows up to 90% of DNA recovery.įigure: PCR fragments of 300 bp were purified from 40 μL of a PCR stock solution using FastGene ® Gel/ PCR Extraction Kit and a competitor kit, according to manufacturers protocol. Everything you need to cut out your DNA fragments. ![]() Extra benefitsĮach FastGene ® Gel/PCR Extraction Kit includes 5 FastGene ® Agarose Gel Band Cutters and 50 µL Midori Green Advance DNA stain (enough to stain 1 L of agarose gels). ![]() With a simple and fast bind-wash-elute procedure you can purify DNA ranging from 15 bp to 10 kb with an elution volume of 20-50 µL. The FastGene ® Gel/PCR Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from agarose gels and PCR products. DNA fragments purified with FastGene ® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, restriction digestion, microarray analysis, PCR and in vitro transcription. The FastGene ® Gel/PCR Extraction Kit is designed for the extraction of DNA from agarose gels and for the purification of PCR products. Details Two in one – DNA cleanup from agarose gels and PCR
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![]() ![]() Razer Orbweaver Chroma Keypad UK - US - Razer Tartarus UK - US - Currency Donations welcome Crypto Donations welcome Ethereum Donation Welcome 0xa970Cbd1c98cf46a8A561590487D1E5898167Ae4 GBX Donation Weclome GLSFvczyrsX8EaJPu26GMoesSbmG83H6Ew Vertcoin Donations Welcome VmQSESx26D22JFqmt7eCbEdnqpdq5M5k34 Vivo Donations Weclome VLVzBCsEcQ1gRuNhrBAA1D3cyc5V4DfZun - Post Items to Beardedbob Studio Unit 15596 Courier Point 13 Freeland Park Wareham Road Poole Dorset BH16 6FH UK. From preloaded lighting effects for different types of games to your own custom uniquely programmed palette of colors you can effortlessly enhance your gaming experience in a way that is unique only to you. CUSTOMIZABLE RAZER CHROMA LIGHTING The Razer Orbweaver Chroma features individually programmable backlit keys with 16.8 million color options all easily set through Razer Synapse. Slide either part back or forth so that it sits under your thumb or cradles your palm perfectly reducing hand fatigue and optimizing ergonomic access to your controls. CUSTOMIZE YOUR WAY The Razer Orbweaver Chroma was designed with adjustable hand thumb and palm-rest modules to let you customize the most comfortable experience with the keypad during marathon gaming sessions. ![]() TOURNAMENT-GRADE PRECISION Designed from the ground up specifically for gaming the Multi-award winning Razer Mechanical Switches have an optimized set of actuation and reset points that improve your gaming performance by giving you speed and responsiveness like never before. Get access to infinite commands and macros while enjoying the same clicky satisfaction as a mechanical keyboard. From preloaded lighting effects for different types of games, to your own custom uniquely programmed palette of colors, you can effortlessly enhance your gaming experience in a way that is unique only to you.Razer Orbweaver Chroma Stealth Review Gaming Keypad #razer #razerorbweaver #keypad #review #gamingHardware #techtuesdays #beardedbob This is a video on my setup for the Razer Orbweaver Chroma Stealth Review Gaming Keypad The Razer Orbweaver Chroma gives you total control in the palm of your hand. The Razer Orbweaver Chroma features individually programmable backlit keys with 16.8 million color options, all easily set through Razer Synapse. Chroma backlighting with 16.8 million customizable color options Slide either part back or forth so that it sits under your thumb or cradles your palm perfectly, reducing hand fatigue, and optimizing ergonomic access to your controls. The Razer Orbweaver Chroma was designed with adjustable, hand, thumb and palm-rest modules to let you customize the most comfortable experience with the keypad during marathon gaming sessions. Adjustable hand, thumb, and palm-rest modules The programmable 8-way directional thumb-pad can also be used either for movement or as modifier keys to expand your repertoire of game-destroying abilities even further. 30 fully programmable keys including an 8-way thumb-padĪrm yourself with 20 fully programmable mechanical keys for more skills, hotkeys, and macros right at your fingertips. ![]() Multi-award winning Razer Mechanical Switchesĭesigned from the ground up specifically for gaming, the Multi-award winning Razer Mechanical Switches have an optimized set of actuation and reset points that improve your gaming performance by giving you speed and responsiveness like never before. It all feels rather leisurely and uncomplicated. It’s not an experience heavy on grind and you can max out your talent tree with ease. It’s nice not to worry so much about experience, although that also allows you to boost your core attributes and pick a talent. If you attempt to pick locks, that will increase also. If you block a lot, your block stat goes up. Your skills increase the more actions you perform. Things do get tougher as you go but the levelling system helps you keep pace. I should note I played it on the default difficulty but there’s plenty of room for increased challenge in the settings. Most of the shields felt reliable and, as long as you weren’t knocked down, you could tank hits. ![]() I did find enemy AI to be a predictable and easy enough to manipulate. I tried to keep fights one-on-one wherever possible. It didn’t cause me much bother during my playthrough although mobs pose a greater threat than solitary encounters. I ended up cowering behind my shield but it’s nice to have those evasive manoeuvres at your disposal.Ĭontrols feel reliable and combat is straight-forward. There’s also a dodge button which allows you to sidestep hits. You have a jump button which does come in handy for some easy platforming sections. It’s a shame that versatility is missing but there’s still plenty of ways to mix and match your gear. Shields and armour have set defensive values and it appears bows can only substitute a main weapon, rather than exist as an off-hand option. You can hold weapons in your right hand and shields in your left and the controller’s trigger correspond to those hands. It’s real-time combat and whilst your weaponry has a wide damage range, you can reliably whittle enemies down without worrying too much about fate dulling your attacks. Melee combat is kept simple with swords, maces and crossbows making up most of your arsenal. If this is your first entry to the series, the intro sets the scene up well enough.Īs an RPG, Ravensword: Shadowlands isn’t the deepest. I don’t mind it but it offers little in the way of intrigue. Characters from previous games in the series turn up but, mostly, people exist to give you quests rather than backstory. There rest of the narrative is slender and serves mostly to give the world just a tiny bit of colour. The opening battle does give you the opportunity to create your character but mine spent most of his time clad in armour. You’re chosen as the person to stop this and need to find the titular Ravensword to turn the tide of war. The kingdom of Tyreas is in trouble with the demonic Ul Thok looking for control. As our hero, you begin as a lone survivor of a bloody battle against the invading Dark Elves. Ravensword: Shadowlands’ story is a basic enough tale. It isn’t pretty or clever but I did find myself enjoying it. Ravensword: Shadowlands is now on consoles and it seems Crescent Moon Games have opted for a port rather than a more explicitly updated effort. I’m not complaining but I did not expect to review a re-release of a game that first saw daylight in 2012 on mobile platforms. It seems like anything will find its way into the Playstation store. ![]() Apin PS4 / Reviews tagged crescent moon games / dark elves / giants / mages / ravensword shadowlands / role-playing game / shield / sword / trolls by Mike ![]() ![]() Does this report consider the impact of COVID-19 and the Russia-Ukraine war on the Pest Control market?.Geographically, the detailed analysis of consumption, revenue, market share and growth rate, historical data and forecast (2018-2029): Inquire or Share Your Questions If Any before the Purchasing This Report: The report also presents the impact of regional conflict on this market in an effort to aid the readers to understand how the market has been adversely influenced and how it’s going to evolve in the years to come.įactors that may help create opportunities and boost profits for market players, as well as challenges that may restrain or even pose a threat to the development of the players, are revealed in the report, which can shed a light on strategic decisions and implementation. Other aspects, such as changes in consumer behavior, demand, transport capacity, trade flow under COVID-19, have also been taken into consideration during the process of the research. The report describes the market scenario during and post the pandemic in the vision of upstream raw materials, major market participants, downstream major customers, etc. Get a Sample Copy of the Pest Control Market ReportĪmid the COVID-19 crisis, the Pest Control market has definitely taken a hit. Major Players in Pest Control market are: The global Pest Control market size was valued at USD 4509.0 million in 2022 and is expected to expand at a CAGR of 12.69 percentage during the forecast period, reaching USD 9232.24 million by 2028. ![]() Market Analysis and Insights: Global Pest Control Market Also, the effects of COVID-19, rising market trends, mergers and acquisitions, and regional conflicts will all be taken into account. The study introduces competitors in the industry and details the top businesses from the standpoint of market share, market size, growth rate etc. These classifications are further broken down into divisions in order to provide a deeper understanding of the Pest Control market. Global “ Pest Control Market” Research are detailed by types, applications, and geographical. "Final Report will add the analysis of the impact of COVID-19 on this industry. Pages Pest Control market report is segmented by geographical regions (Europe, Asia Pacific, Latin America, Middle East & Africa) and manufactures (Harris, Bayer Advanced, Spectrum Brands, Ortho, Rollins, BASF, Ecolab, Garden Tech, Terminix, SC Johnson, Massey Services, Anticimex, Kill germs, MGK, Rentokil Initial, Willert Home Products, Bonide Products-). ![]() Oh God! May You be the One who is increasing in our lives! May we continue to set our hearts on You and allow You to enable us to put the flesh to death. Maybe it’s time to ask ourselves some questions: Is Jesus increasing in authority and prominence in my life? Is He the One people think of when they meet me or we part ways? Are people being attracted to Him through my broken life? Am I experiencing a deeper and fuller understanding of His goodness and grace, even in the midst of great hardship and pain? The opposite questions also reveal who is increasing and who is decreasing. This revealing (and the ensuing humble submission on my part that should follow) sets up the win-win situation where my flesh decreases (a VERY good thing for me and everyone else!) and His authority, power, and grace in me and upon me increase. Trials reveal our weaknesses and our inabilities, but they also reveal His strength and His capability. Why? Because He uses these things to strip away the material and superficial layers of me and causes my heart to be tender and raw to Him in a way that is seemingly not possible when everything is fine and dandy. The thought struck me this morning that the reality of this verse really pops out when we get into trials, struggles, and suffering. Even in our discussions we groan inwardly about having to die daily, having to give up our way and our will. At the same time, He is indwelling me with His Spirit and enabling me to be absorbed by Him – the old man is crucified and I am raised to life with Christ (Galatians 2:20). We must see that for every decrease of Self, Christ is increased. This drives me to prayer and to the Word of God and to waiting on the Lord His ways and His character and His honor become my desire and my focus, over and above any and every other wish, dream, desire, and plan. Instead of seeking my own selfish gain or merely looking to help those around me, my first concern must be my Master’s glory and will. What does it mean that He increase and I decrease in our personal context? For starters, it means that we must be absorbed with and by Him. John’s words should pierce our prideful and self-seeking hearts and cause us to pause in brokenness and awe. It is here that the “rubber hits the road”, so to speak. It is not just a great concept and a lofty, but improbable goal no, our decrease and His increase is what dying to self and living to God is all about. Here within this verse John the Baptist expressed what is perhaps the preeminent attitude that is necessary for us to truly follow Christ. The more He grows in us, the more we fulfil our potential and become all that God wants us to be without ceasing to be ourselves.“He must increase, but I must decrease.” ~John 3:30 We can also say, “He must increase I must decrease'' as His presence increases in us and our ego welcomes Him. The closer we come to Jesus, the more we know how much we need to grow. None of those things applied to John the Baptist and recognised the uniqueness of Jesus, which is why he could say, “he must increase, but I must decrease.” We cannot fully appreciate the mystery of Jesus, if we do not allow the influence of Christ to dominate in that of the self in us. ![]() With these two - Jesus and the Holy Spirit - as official witnesses, the Truth cannot be denied: God so loves the world that God sent the only begotten Son so that all those who believe in Him might have eternal life and those who refuse to believe, after they have been given the opportunity to believe, will experience God’s wrath. Not only did Jesus give witness to the love which God has for the people, but also the Holy Spirit gives witness to the eternal life which is the gift for all those who believe. Jesus announces that the One “from above” (ἄνωθεν - anothen) can speak of His experience of God because He has been with God. Jesus is aware that He is from "above” and that the Father has entrusted everything to Him. In today's Gospel, Jesus tells Nicodemus that He must continue to speak about the relationship He has with His Father because He has been empowered by the Holy Spirit for this very task from above. The key is discipline of mind, body and spirit. If you have weight problems of any kind, this book is for you Inside is 20 years experience in counseling men/women with weight control problems. It is not just the apostles and disciples who testified that Jesus died and rose for the forgiveness of our sin the Holy Spirit – God’s divine Spirit – is also testifying to the Truth. He Must Increase but I Must Decrease is for those who overweight, underweight, bulemic or anorexic. Peter replies that they must give witness to the truth of God, by and through the power of the Holy Spirit who raised Jesus from the dead. For Peter, obedience to God’s command is more important than obeying the orders of mortals, even if they are the religious authorities. Today's first reading relates the experience of Peter and John before the Sanhedrin as they were accused of disobeying the council’s orders. The Sanhedrin clearly made known to them that they should stop to preach in the Name of the Lord Jesus, crucified and alleged to be risen from the dead. This means that your podcast will potentially appear in people’s search results. But if it’s not, adding it to Google Podcasts allows your podcast to be crawled and indexed by Google Search. Your podcast feed may already be on Google Podcasts, which means that you only have to claim it to access Google Podcast Manager. This could help you understand if your podcast is too long or if there are segments that people don’t find particularly interesting. When listeners stop listening: Discover how far listeners get into each episode, including the exact moment they drop off.Top-discovered episodes: Find out information on your top-performing episodes, which could help you choose more winning topics for future podcast episodes.This shows what your podcast ranks well for, which can help you improve your SEO (search engine optimization). Search terms: What keywords people use to search.Clicks: Of those impressions, how many people actually clicked through to the podcast or episode page.For example, if they searched “mountain climbing podcast” and your podcast came up in the search results that they saw, they got an “impression” of your podcast. Impressions: How often a listener sees a link to your show or a specific episode in a Google search.Once you claim your podcast, you’ll see data points such as: ![]() It should be no surprise that a Google-backed podcast manager will give you a wealth of information on how listeners find and consume your show. It takes very little effort to add your show to Google (you just have to claim your RSS feed), and once you do, you can reap a ton of benefits. And even better, this service is completely free. Google Podcast Manager gives you more information about your podcast and your listeners’ habits that you can use to improve future episodes and get more engagement from your audience. Keywords listeners used to discover your show.How much of your latest episode people listened to and when they tended to drop off.How many listens or downloads your podcast receives on the Google Podcast app. ![]() Once you have claimed your podcast on Google Podcast Manager, you will see a range of data, including: This means that Google Podcast Manager will give you insights into the listeners who tune in via the Google Podcast app along with how your podcast performs in Google Search. Google Podcast Manager is a feature from Google that gives you customized data about how your podcast is performing across Google’s properties. |